The crowd as a cameraman : on-stage display of crowdsourced mobile video at large-scale events

Recording videos with smartphones at large-scale events such as concerts and festivals is very common nowadays. These videos register the atmosphere of the event as it is experienced by the crowd and offer a perspective that is hard to capture by the professional cameras installed throughout the venue. In this article, we present a framework to collect videos from smartphones in the public and blend these into a mosaic that can be readily mixed with professional camera footage and shown on displays during the event. The video upload is prioritized by matching requests of the event director with video metadata, while taking into account the available wireless network capacity. The proposed framework's main novelty is its scalability, supporting the real-time transmission, processing and display of videos recorded by hundreds of simultaneous users in ultra-dense Wi-Fi environments, as well as its proven integration in commercial production environments. The framework has been extensively validated in a controlled lab setting with up to 1 000 clients as well as in a field trial where 1 183 videos were collected from 135 participants recruited from an audience of 8 050 people. 90 % of those videos were uploaded within 6.8 minutes

Hierarchical browsing of digital video sequences

C

Multi-Step Media Adaptation: Implementation of a Knowledge-Based Engine

Continuing changes in the domains of consumer devices and multimedia formats demand for a new approach to media adaptation. The publication of customized content on a device requires an automatic adaptation engine that takes into account the specifications of both the device and the material to be published. These specifications can be expressed using a single domain ontology that describes the concepts of the media adaptation domain. In this document, we provide insight into the implementation of an adaptation engine that exploits this domain knowledge. We explain how this engine, through the use of description matching and Semantic Web Services, composes a chain of adaptation services which will alter the original content to the needs of the target device

Epigenomic analyses in sub-populations of spermatozoa from infertile men with short anogenital distance

The key developmental steps in the male germ cell lineage include the epigenetic reprogramming of the primordial germ cells, the large-scale replacement of histone by protamine in spermatids and some degree of apoptosis. In past experiments, we prenatally exposed mice to di(2-ethylhexyl)phthalate (DEHP, CAS number 117-81-7). At adulthood, the exposed mice were characterized by reduced anogenital distance (AGD), poor semen quality, and epigenomic abnormalities in their gametes' DNA. However, the phenotype heavily depended on the genetic background of the mouse strain. We identified SNP-dependent binding motifs for sex hormones signaling pathways varying between the susceptible and the resistant mouse strains. These SNP-dependent motifs were located in the genes that were the most affected one by DEHP, at both DNA methylation and RNA transcription levels. We then tried to replicate these observations into human. We selected 6 infertile patients with short AGD and 5 fertile sperm donors. Testicular volume was significantly reduced in infertile men with shorter AGD. Sub-populations of spermatozoa were FACS-sorted using the chromomycin A3 dye (protamine vs. histone) and with the YO-PRO-1 dye (apoptotic vs. non-apoptotic). In the four sorted sperm populations, we analyzed the epigenomes through reduced-representation bisulfite sequencing (RRBS). The results demonstrated both lower CpG methylation and higher DNA fragmentation in infertile patients. Interestingly, numerous de-methylated regions localized within families of Alu repeats containing estrogen response elements (ERE). We are now in the process of replicating the entire study with a new cohort of five infertile patients and five fertile controls. The aim is to understand if there is a relationship between these epigenomic alterations within Alu repeats containing ERE and fertility, and whether exposures to anti-androgenic endocrine disruptors may alter methylation marks within these Alu-ERE repeats

Altered DNA methylation in estrogen-responsive repetitive sequences of spermatozoa of infertile men with shortened anogenital distance

Background: It has been suggested that antenatal exposure to environmental endocrine disruptors is responsible for adverse trends in male reproductive health, including male infertility, impaired semen quality, cryptorchidism and testicular cancer, a condition known as testicular dysgenesis syndrome. Anogenital distance (AGD) is an anthropomorphic measure of antenatal exposure to endocrine disruptors, with higher exposure levels leading to shortened AGD. We hypothesized that exposure to endocrine disruptors could lead to changes in DNA methylation during early embryonic development, which could then persist in the sperm of infertile men with shortened AGD. Results: Using fluorescence activated cell sorting based on staining with either YO-PRO-1 (YOPRO) or chromomycin-3 (CMA3), we isolated four sperm fractions from eleven infertile men with short AGD and ten healthy semen donors. We examined DNA methylation in these sorted spermatozoa using reduced representation bisulfite sequencing. We found that fractions of spermatozoa from infertile men stained with CMA3 or YOPRO were more likely to contain transposable elements harboring an estrogen receptor response element (ERE). Abnormal sperm (as judged by high CMA3 or YOPRO staining) from infertile men shows substantial hypomethylation in estrogenic Alu sequences. Conversely, normal sperm fractions (as judged by low CMA3 or YO-PRO-1 staining) of either healthy donors or infertile patients were more likely to contain hypermethylated Alu sequences with ERE. Conclusions: Shortened AGD, as related to previous exposure to endocrine disruptors, and male infertility are accompanied by increased presence of hormonal response elements in the differentially methylated regulatory sequences of the genome of sperm fractions characterized by chromatin decondensation and apoptosis.</p